Integrated Metabolomic and Transcriptomic Analysis Decodes Heat Stress-Induced Metabolic Shifts in Gilt Granulosa Cells.

ABSTRACT: While previous studies have extensively demonstrated that summer heat stress (HS) impairs oocyte quality via follicular granulosa cell (GC) mediation, the molecular mechanisms underlying HS-induced GC-mediated oocyte damage-particularly at the multi-omics level-remain poorly understood. This study integrated metabolomic and transcriptomic analyses of GCs from gilts under seasonal thermal stress (winter CON vs. summer HS) to elucidate GC-mediated regulatory networks affecting oocyte quality. Non-targeted metabolomics identified 45 differentially accumulated metabolites (DAMs, p < 0.05), with 69% being lipids/lipid-like molecules enriched in pathways such as glycerophospholipid metabolism, choline metabolism, linoleic acid metabolism, the adipocytokine signaling pathway, and the sphin

SUBMITTER: Tang P 

PROVIDER: S-EPMC12656734 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature