Nucleoredoxin 1 in Wheat: Genomic Analysis and Demonstration of Its Role in Redox Homeostasis and Stress Resilience.

ABSTRACT: Nucleoredoxin 1 (NRX1), a member of the redoxin superfamily, plays a critical role in maintaining redox homeostasis and enhancing stress tolerance in plants. We employed integrated in silico analyses and CRISPR-Cas9-based genome editing to functionally characterize NRX1 in Triticum aestivum (wheat) responding to salinity and infection by Puccinia striiformis. We identified five NRX1 proteins coded by three homeologs, with each containing conserved thioredoxin-like domains and a Cys-rich C-terminal region. Sequence analysis predicted cytosolic and chloroplast localization, and promoter analysis predicted interaction with numerous cis-regulatory elements responsive to stress and hormones, including ABRE, MeJARE, and LTRE motifs. Expression profiling revealed significant

SUBMITTER: Ghafoor MS 

PROVIDER: S-EPMC12688404 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature