Extracellular Vesicles from <i>Lactobacillus rhamnosus BS-Pro-08</i>, Kefir Grain, Suppress Adipogenesis and Enhance Lipolysis in Adipocytes.

ABSTRACT: Extracellular vesicles (EVs) derived from probiotic bacteria have recently emerged as postbiotic mediators that regulate host cellular responses. This study investigated the effects of EVs from Lactobacillus rhamnosus BS-Pro-08, isolated from kefir grains (Lacto EV), on adipocyte differentiation and lipid metabolism. Lacto EV treatment markedly suppressed the differentiation of 3T3-L1 preadipocytes into mature adipocytes, as reflected by reduced lipid accumulation and decreased expression of the adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). This inhibitory effect was most pronounced at the early stage of adipogenesis. In mature adipocytes, Lacto EV enhanced lipolysis in a dose-dependent manner, ac

SUBMITTER: Park BO 

PROVIDER: S-EPMC12692708 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature