Accelerating AAV purification process development using high-throughput resin tip module.

ABSTRACT: Recombinant adeno-associated viruses (AAVs) with precise genome editing and cell-virus interaction have become a promising delivery tool for gene therapy. A robust AAV purification process is crucial for ensuring therapeutic efficacy. The challenges of AAV purification process development encompass limited material availability during early-stage development, high cost-of-goods compared to traditional biologics, and short development timelines for the critical first-in-human stages. The key to overcoming these challenges is to leverage high throughput (HTP) methods. In this article, an integrated end-to-end HTP workflow is proposed, utilizing a resin tip as the purification module and incorporating an HTP analytical toolkit on one platform. Purification parameters, including binding capaci

SUBMITTER: Li Q 

PROVIDER: S-EPMC12696448 | biostudies-literature | 2025 Nov-Dec

REPOSITORIES: biostudies-literature