Uterine histotroph and conceptus development. V. Comparative analyses of arginine-mediated transcriptomic reprogramming in conceptus trophectoderm of pigs and sheep†.

ABSTRACT: In ungulates such as pigs and sheep, blastocyst elongation is essential for implantation, driven by coordinated trophoblast proliferation, cytoskeletal remodeling, and nutrient signaling. L-arginine (Arg), a conditionally essential amino acid enriched in uterine histotroph during early pregnancy, plays a key role in conceptus development; however, its genomic effects on the trophectoderm are poorly defined. Therefore, we investigated Arg-responsive transcriptomic changes in porcine (pTr2) and ovine (oTr1) trophectoderm cells using RNA-seq and integrative pathway analyses. Arg significantly stimulated proliferation in both cell types in a non-linear, dose-dependent manner, with maximal effects at 0.2 mM, consistent with physiological levels. Transcriptomic profiling identified 2723 and 5369

SUBMITTER: Paudel S 

PROVIDER: S-EPMC12706476 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature