Proteomic Analysis of the Differential Response of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> to <i>Lacticaseibacillus rhamnosus</i> Cell-Free Supernatant and Lactic Acid.

ABSTRACT: Background/Objectives: Postbiotics derived from lactic acid bacteria are emerging as promising antimicrobial agents due to their antibacterial, antibiofilm, and immunomodulatory properties. Among their metabolites, lactic acid (LA) is thought to play a major role in antimicrobial activity. This study investigated the proteomic response of Pseudomonas aeruginosa and Staphylococcus aureus to Lacticaseibacillus rhamnosus cell-free supernatant (CFS) and compared it with that elicited by LA alone. Methods: Overnight bacterial cultures were exposed to sub-MIC LA or CFS (1:10 for P. aeruginosa and 1:8 for S. aureus; ~12.5-15.6 mM LA) for 6 h at 37 °C. Intracellular proteins were harvested and subsequently quantified and purified to be analysed by H

SUBMITTER: Bianchi M 

PROVIDER: S-EPMC12730115 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature