Comparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of <i>Cistanche</i>.

ABSTRACT: This study focuses on polysaccharides from Cistanche deserticola and Cistanche tubulosa, medicinal plants renowned for their health benefits. The "water extraction and alcohol precipitation" method was used to obtain the crude polysaccharides of the wine-making residues of C. deserticola (CDP) and C. tubulosa (CTP), respectively. Then, ultrafiltration membrane (UFM), DEAE-52, and Sephadex-G75 or Smartdex-G100 gel chromatography were used to separate and purify the crude polysaccharides, yielding the homogeneous fractions CDP1-5-1, CDP2-2-2, CDP2-3-2, CTP1-5-1, and CTP1-5-3. Structural analysis was conducted by using Fourier-transform infrared spectroscopy (FT-IR), high-performance anion-exchange chromatography coupled with mult

SUBMITTER: Ruan J 

PROVIDER: S-EPMC12736087 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature