[He<b>'</b>s Yangchao recipe ameliorates premature ovarian insuf-ficiency by remodeling intestinal microbiota to promote granulosa cell glycolysis].

ABSTRACT:

Objectives

To investigate the molecular mechanism by which He's Yangchao recipe improves ovarian function in premature ovarian insufficiency (POI) mice through intestinal flora modulation.

Methods

Forty female ICR mice (aged 6-8 weeks) were intraperitoneally injected with cyclophosphamide (150 mg/kg) to establish a POI model, while 10 untreated mice served as the blank control. The cyclophosphamide-injected mice were randomly divided into four groups with 10 mice in each group: low-dose He's Yangchao recipe (crude herb, 6 g/kg), high-dose He's Yangchao recipe (crude herb, 25 g/kg), estradiol (positive control), and model control (distilled water). Treatments were administered daily by gavage for 6 weeks. Vaginal exfoliated cells were stained with Wright-Giemsa solution to mo

SUBMITTER: Lin F 

PROVIDER: S-EPMC12750045 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature