METTL3-Mediated m6A Modification of DSC1 Alleviates Inflammation and Cellular Dysfunction in Periodontitis.

ABSTRACT:

Background

The inflammation and cellular dysfunction in periodontitis largely depend on N6-methyladenosine (m⁶A) modification-related epigenetics. However, the role of methyltransferase-like 3 (METTL3)-differentiated m⁶A modification of desmocollin 1 (DSC1) in periodontitis remains to be investigated.

Methods

Periodontal ligament (PDL) tissues were collected from patients with periodontitis and healthy volunteers, and a periodontitis model was constructed using lipopolysaccharide (LPS) and human periodontal ligament fibroblasts (HPLF). METTL3 and DSC1 levels in the specimens and cells were measured using quantitative reverse-transcription polymerase chain reaction. Proliferation, apoptosis, and inflammatory changes were analyzed using CCK-8, EdU, flow c

SUBMITTER: Su Q 

PROVIDER: S-EPMC12800397 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature