Anionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum Immunoglobulins.

ABSTRACT: The detailed characterization of antigen-specific serum antibodies is hindered by the lack of efficient, gentle isolation methods. In this context, standard column affinity chromatography, although a powerful purification tool, presents practical challenges, including high antigen consumption and elution conditions that risk inducing antibody polyreactivity, while conventional acidic elution often compromises antibody integrity. This study introduces a novel microscale method for isolating specific immunoglobulins using anionic detergents as mild eluents. We employed antigen-functionalized hydrogel microarrays and magnetic beads as micro-immunosorbents. Among the tested detergents, sodium lauroyl glutamate (SLG) was optimal, achieving up to 78.3% recovery of functional antibodies. The opti

SUBMITTER: Trukhin D 

PROVIDER: S-EPMC12839167 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature