Long-Term Tolerability and Safety of AAV5-Id3 Gene Therapy to Eyes.

ABSTRACT:

Purpose

The inhibitor of differentiation 3 gene therapy via adeno-associated virus 5 (AAV5-Id3) effectively abrogated corneal fibrosis in vivo. This study examined the long-term tolerability and safety of AAV5-Id3 gene therapy for eyes in vivo using a rabbit model.

Methods

Eighteen New Zealand White rabbits, segregated into three groups (naive, AAV5-naked, and AAV5-Id3; n = 6/group), were used. The clinical eye examinations with slit-lamp and multimodal corneal imaging were performed in live rabbits periodically to record the status of ocular and corneal health for up to 7 months. Thereafter, humane euthanasia was performed, and cellular and molecular changes in corneas were studied employing histopathologic, immunofluorescence, and quantitative reverse transcription polymer

SUBMITTER: Gupta S 

PROVIDER: S-EPMC12859705 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature