Menstrual blood-derived mesenchymal stromal cell secretome modulates macrophage polarization in a preconditioning-dependent manner.

ABSTRACT:

Background

The effects of menstrual blood-derived mesenchymal stromal cell secretome (S-MenSC) on macrophage polarization remain unclear. This study studied the impact of secretomes from basal MenSCs (S-bMenSCs) and those preconditioned with IFNγ and TNFα (S-pMenSCs) on human monocytes and macrophages in vitro.

Methods

S-MenSCs were used to assess their effects on three stages of monocyte-derived cell maturation: i. monocyte differentiation; ii. polarization of monocyte-derived macrophages (MDMs) toward M1-like or M2-like phenotypes; and iii. reprogramming of pre-polarized M1 or M2 macrophages. Surface markers were analyzed by flow cytometry and cytokine gene expression by RT-qPCR. In addition, a proteomic profiling was performed to identify proteins involved in the o

SUBMITTER: de Pedro MA 

PROVIDER: S-EPMC12872789 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature