Enrichment of Cysteine S-palmitoylated Peptides Using Sodium Deoxycholate Acid Precipitation.

ABSTRACT: S-palmitoylation is a poorly understood post-translational modification that is gaining more attention as an essential regulator of cellular processes. The reversible nature of S-palmitoylation may allow for fine-tuned control of cellular events and adaptation to stimuli. The detection of S-palmitoylated proteins and peptides includes the Acyl-Biotin Exchange (ABE) method, Acyl resin-assisted Capture (Acyl-RAC), metabolic labelling, and derivatives thereof. We present a novel method of enrichment of S-palmitoylated peptides termed SDC Acid Precipitation Enrichment (SDC-ACE). Here, S-palmitoylated peptides are enriched by taking advantage of their co-precipitation with Sodium deoxycholate (SDC) under acidic conditions, allowing easy and fast separation of lipidated peptides from the sample

SUBMITTER: Jensen PT 

PROVIDER: S-EPMC12874130 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature