Differential effect of Periodontal pathogen <i>Fusobacterium nucleatum</i> and <i>Porphyromonas gingivalis</i> on modulation of specific partial-EMT genes in colorectal cancer cells.

ABSTRACT:

Objective

While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of Fusobacterium nucleatum (Fn) and Porphyromonas gingivalis (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).

Materials and methods

HCT 116 cells were infected with Fn or Pg at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate

SUBMITTER: Gopinath D 

PROVIDER: S-EPMC12907499 | biostudies-literature | 2026 Mar-Apr

REPOSITORIES: biostudies-literature