Dysregulation of YTHDF2 promotes the over expression of interferon beta in anti-MDA5 antibody-positive dermatomyositis.

ABSTRACT:

Background

Dysregulation of N6-methyladenosine (m6A) has been implicated in the pathophysiology of various autoimmune diseases. However, its role in dermatomyositis (DM), particularly in cases associated with anti-MDA5 antibodies, remains unclear. This study aimed to elucidate the potential involvement of m6A modifications of mRNAs in the pathogenesis of anti-MDA5⁺DM.

Methods

We assessed mRNA m6A methylation levels in peripheral blood mononuclear cells (PBMCs) from anti-MDA5⁺DM patients and healthy controls (HC) using LC-MS/MS assay. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting were conducted to determine the mRNA and protein expression levels of YTHDF2 in PBMCs and monocytes from anti-MDA5⁺DM pa

SUBMITTER: Zhang M 

PROVIDER: S-EPMC12911376 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature