Conjugates of α-d-Galp-(1→3)-β-d-Galp for the serological diagnosis of Chagas disease.

ABSTRACT:

Background

Chagas disease (ChD), caused by the parasitic protozoan Trypanosoma cruzi, is a lifelong, neglected tropical disease with substantial medical and socioeconomic impact. Despite this situation, currently available diagnostic and therapeutic methods display serious limitations. A promising strategy to improve ChD serodiagnosis involves targeting parasite carbohydrate antigens, particularly the α-galactosyl-rich mucins that coat the surface of bloodstream trypomastigotes (tGPI-mucins).

Methods/principle findings

Here, we present a concise and efficient protocol for the chemical synthesis of a tGPI-mucin-derived glycotope, the disaccharide α-D-Galp-(1 → 3)-β-D-Galp, and its functional conjugation to different scaffolds using the squarate method. A neoglycoprotein made

SUBMITTER: Balouz V 

PROVIDER: S-EPMC12915924 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature