Integrative transcriptomic and metabolomic analyses provide insights into the effects of overexpression and knockout of <i>NtLHT1</i> in different tissues.

ABSTRACT: Lysine-Histidine Transporter 1 (LHT1) is a key amino acid transporter involved in plant nutrition, metabolism, leaf development, and abiotic stress tolerance. However, the regulatory mechanisms of NtLHT1 (LHT1 in Nicotiana tobacum) across different plant organs remain poorly understood. In this study, we utilized NtLHT1 knockout (LHT1-KO), overexpression (LHT1-OE), and wild-type (LHT1-WT) tobacco lines (cv. Honghuadajinyuan) to investigate the organ-specific effects of altered NtLHT1 expression on metabolic and transcriptomic profiles. At the 10-leaf stage, leaves, stems, and roots were collected for transcriptome sequencing (RNA-seq) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based metabolomic analysis, with qRT-PCR performed to

SUBMITTER: Xing J 

PROVIDER: S-EPMC12920487 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature