Combined Serum (1,3)-β-D-Glucan and Oral Wash PCR as a Noninvasive Diagnostic Strategy for Early Detection of <i>Pneumocystis jirovecii</i> Pneumonia: An Observational Retrospective Study.

ABSTRACT:

Background

The diagnosis of P. jirovecii pneumonia (PJP) in immunosuppressed, HIV-negative patients is often limited by the inability to perform a bronchoalveolar lavage (BAL) due to respiratory insufficiency. In this study, we evaluated the diagnostic accuracy of combining serum beta-D-glucan (BDG) testing with P. jirovecii polymerase chain reaction (PCR) in oral wash (OW) samples as noninvasive tools for PJP diagnosis.

Methods

This was a retrospective, single-center study including all immunosuppressed non-HIV adult patients with suspected PJP in whom a Fujifilm Wako β-glucan test BDG and BAL PJ in-house PCR determination were performed during a 5-year period (2019-2024). The diagnostic performance (negative predictive value [NPV], positive predictive value [

SUBMITTER: Falco-Roget A 

PROVIDER: S-EPMC12923328 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature