Loop-Mediated Isothermal Amplification Enables Reliable Kaposi Sarcoma Diagnosis Across Time and Sites in East Africa.

ABSTRACT: Kaposi sarcoma (KS) is an endothelial cancer caused by the Kaposi sarcoma-associated herpesvirus (KSHV), and it remains one of the most frequently diagnosed and fatal cancers in sub-Saharan Africa. Achieving timely and accurate diagnosis for KS is a critical challenge in sub-Saharan Africa, where access to histopathology is limited and clinical diagnosis is often erroneous. Previously, a point-of-care device was developed to quantify KSHV DNA and demonstrated promising performance, achieving 97% sensitivity and 92% specificity at a 26-min threshold in a training set of patients from several Ugandan clinics. Herein, 421 skin biopsies from a different, test set of patients identified later in calendar time at the same clinical sites were examined using the same methodology. Using the previou

SUBMITTER: Chu X 

PROVIDER: S-EPMC12924702 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature