Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes.

ABSTRACT: Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.

SUBMITTER: Kumar A 

PROVIDER: S-EPMC12925548 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature