Quercetin-loaded silicon dioxide-graphene nanoparticles promotes M2 macrophage reprogramming in mycoplasma-induced pneumonia.

ABSTRACT:

Objective

To develop and evaluate a quercetin-loaded silicon dioxide-graphene nanocomposite (Que@SiO₂-GNPs) for targeted macrophage modulation and epithelial protection in a Mycoplasma-induced pneumonia model.

Methods

Que@SiO₂-GNPs were synthesized via graphene oxide dispersion, sol-gel silica coating, and quercetin adsorption. Physicochemical properties were characterized by XPS, XRD, and FTIR. The therapeutic efficacy was evaluated in MP-infected rat models via histopathology, ELISA, flow cytometry, immunostaining, and Western blot. Cellular uptake, polarization, and functional assays were performed in primary AMs and BEAS-2B epithelial cells.

Results

Que@SiO₂-GNPs exhibited successful surface modification and quercetin incorporation with stable physicochemical prop

SUBMITTER: Liu X 

PROVIDER: S-EPMC12933981 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature