Proton transfer reagent cations for ion-ion charge state manipulation of high mass negatively-charged analytes in an electrodynamic ion trap.

ABSTRACT: This work summarizes the criteria for a useful reagent for ion/ion proton transfer in an electrodynamic ion trap with specific emphasis on proton transfer to high mass multiply-charged analyte ions. A readily available and ionizable weak base of relatively high mass meets the criteria. The protonated form of an extensively fluorinated 10-carbon primary amine (1H,1H,2H,2H-perfluorodecylamine, PFDA) is demonstrated to serve as a useful reagent for proton transfer to negatively-charged proteins and oligonucleotides. The behavior of protonated PFDA is compared with that of protonated 1,8-bis(dimethylamino)naphthalene (proton sponge) with respect to the tendency for proton transfer versus attachment in ion/ion reactions with a common multiply-deprotonated prot

SUBMITTER: Ellin NR 

PROVIDER: S-EPMC12935111 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature