Using FASS-LTP in postmortem mice brain tissues to assess pathological synaptic function.

ABSTRACT:

Background

Study of synaptic integrity using conventional electrophysiology is a gold standard for quantitative assessment of neurodegeneration. Fluorescence assisted single-synapse long-term potentiation (FASS-LTP) provides a high throughput method to assess the synaptic integrity of neurotransmission within and between different brain regions as a measure of pharmacological efficacy in translational models.

New method

We adapted the existing method to our purpose by adding a step during the thawing of frozen samples, by an extra step of placing them on a rocker at room temperature for 30 minutes immediately following thawing with constant mixing on a shaker. This allowed for gradual, uniform thawing, effectively separating the synaptosomes. Our study demonstrates FASS-LTP

SUBMITTER: Currie J 

PROVIDER: S-EPMC12940509 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature