Simultaneous Quantification of Fumonisins and Their Hydrolyzed Metabolites in Donkey Matrices: A Tool for Exposure Assessment and Toxicokinetic Studies.

ABSTRACT: A novel, sensitive, and robust LC-MS/MS method was developed and fully validated for the simultaneous determination of fumonisins (FB₁, FB₂, FB₃) and their hydrolyzed metabolites (HFB₁, HFB₂, HFB₃) in donkey plasma, urine, and feces-three critical matrices for toxicokinetic studies. Sample preparation was optimized for each matrix: salting-out assisted liquid-liquid extraction (SALLE) with perchloric acidification for urine and feces, and a dilute-evaporate-shoot (DES) approach for plasma. Chromatographic separation was achieved on a BEH C₁₈ column with water-ACN containing 0.5% formic acid. The method demonstrated excellent linearity (R² ≥ 0.99), acceptable accuracy (mean recoveries: 73.3-111.5%), and

SUBMITTER: Tian D 

PROVIDER: S-EPMC12944729 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature