Transcriptional response of steady-state yeast cultures to transient perturbations in carbon source.

ABSTRACT: To understand the dynamics of transcriptional response to changing environments, well defined, easily controlled, and short-term perturbation experiments were undertaken. We subjected steady-state cultures of Saccharomyces cerevisiae in chemostats growing on limiting galactose to two different size pulses of glucose, well known to be a preferred carbon source. Although these pulses were not large enough to change growth rates or cell size, approximately 25% of the genes changed their expression at least 2-fold. Using DNA microarrays to estimate mRNA abundance, we found a number of distinguishable patterns of transcriptional response among the many genes whose expression changed. Many of these genes were already known to be regulated by particular transcription factors; we estimated five po

SUBMITTER: Ronen M 

PROVIDER: S-EPMC1326188 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature