Generation and analysis of expressed sequence tags from NaCl-treated Glycine soja.

ABSTRACT:

Background

Salinization causes negative effects on plant productivity and poses an increasingly serious threat to the sustainability of agriculture. Wild soybean (Glycine soja) can survive in highly saline conditions, therefore provides an ideal candidate plant system for salt tolerance gene mining.

Results

As a first step towards the characterization of genes that contribute to combating salinity stress, we constructed a full-length cDNA library of Glycine soja (50109) leaf treated with 150 mM NaCl, using the SMART technology. Random expressed sequence tag (EST) sequencing of 2,219 clones produced 2,003 cleaned ESTs for gene expression analysis. The average read length of cleaned ESTs was 454 bp, with an average GC content of 40%. These ESTs were assembled using the PHRAP p

SUBMITTER: Ji W 

PROVIDER: S-EPMC1388217 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature