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Genetic screens for mutations affecting development of Xenopus tropicalis.

ABSTRACT: We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We have also demonstrated the feasibility of reverse genetic approaches for obtaining carriers of mutations in specific genes, and have directly determined an induced mutation rate by sequencing specific exons from a mutagenized population. The Xenopus system, with its well-understood embryology, fate map, and gain-of-function approaches, can now be coupled with efficient loss-of-function genetic strategies for vertebrate functional genomics and developmental genetics.

SUBMITTER: Goda T

PROVIDER: S-EPMC1475704 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature

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Genetic screens for mutations affecting development of Xenopus tropicalis.

Goda Tadahiro T Abu-Daya Anita A Carruthers Samantha S Clark Matthew D MD Stemple Derek L DL Zimmerman Lyle B LB

PLoS genetics 20060609 6

We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We ...[more]

PMID: 16789825

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