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A global topology map of the Saccharomyces cerevisiae membrane proteome.

ABSTRACT: The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.

SUBMITTER: Kim H 

PROVIDER: S-EPMC1544055 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature

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A global topology map of the Saccharomyces cerevisiae membrane proteome.

Kim Hyun H   Melén Karin K   Osterberg Marie M   von Heijne Gunnar G  

Proceedings of the National Academy of Sciences of the United States of America 20060717 30

The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryot  ...[more]

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