Expression of P2 receptors in human B cells and Epstein-Barr virus-transformed lymphoblastoid cell lines.

ABSTRACT:

Background

Epstein-Barr virus (EBV) infection immortalizes primary B cells in vitro and generates lymphoblastoid cell lines (LCLs), which are used for several purposes in immunological and genetic studies. Purinergic receptors, consisting of P2X and P2Y, are activated by extracellular nucleotides in most tissues and exert various physiological effects. In B cells, especially EBV-induced LCLs, their expression and function have not been well studied. We investigated the expression of P2 receptors on primary human B cells and LCLs using the quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method for revealing the gene expression profile of the P2 receptor subtypes and their changes during transformation.

Results

The mRNA transcripts of most P2 receptors we

SUBMITTER: Lee DH 

PROVIDER: S-EPMC1609185 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature