Regulation of the alpha-crystallin gene acr2 by the MprAB two-component system of Mycobacterium tuberculosis.

ABSTRACT: Coordinated regulation of molecular chaperones is an important feature of the bacterial stress response. The small molecular chaperone gene acr2 of Mycobacterium tuberculosis is activated by exposure to several stresses, including heat and the detergent sodium dodecyl sulfate (SDS). In this study, we show that acr2 is directly regulated by the MprAB two-component system, and that MprAB has both positive and negative effects on acr2 expression. mRNA analyses showed that acr2 expression levels were lower under SDS stress and control conditions but higher under heat shock in an mprAB deletion mutant than they were in the parental strain. Parental expression patterns were restored in an mprAB-complemented strain. Western blotting using an anti-Acr2 antibody showed that Acr2 protein synthesis c

SUBMITTER: Pang X 

PROVIDER: S-EPMC1951922 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature