Translational genomics to develop a Salmonella enterica serovar Paratyphi A multiplex polymerase chain reaction assay.

ABSTRACT: The use of pathogen genome sequence data for the control and management of infections remains an ongoing challenge. We describe a broadly applicable, web-enabled approach that can be used to develop bacteria-specific polymerase chain reaction (PCR) assays. Salmonella enterica Paratyphi A has emerged as a major cause of enteric fever in Asia. Culture-based diagnosis is slow and frequently negative in patients with suspected typhoid and paratyphoid fever, potentially compromising patient management and public health. We used the MobilomeFINDER web-server to perform in silico subtractive hybridization, thus identifying 43 protein-coding sequences (CDSs) that were present in two Paratyphi A strains but not in other sequenced Salmonella genomes. After exclusion of 29 CDSs found to be variably p

SUBMITTER: Ou HY 

PROVIDER: S-EPMC2049048 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature