Checkpoint proteins control morphogenetic events during DNA replication stress in Saccharomyces cerevisiae.

ABSTRACT: In response to DNA replication stress in Saccharomyces cerevisiae, the DNA replication checkpoint maintains replication fork stability, prevents precocious chromosome segregation, and causes cells to arrest as large-budded cells. The checkpoint kinases Mec1 and Rad53 act in this checkpoint. Treatment of mec1 or rad53Delta mutants with replication inhibitors results in replication fork collapse and inappropriate partitioning of partially replicated chromosomes, leading to cell death. We describe a previously unappreciated function of various replication stress checkpoint proteins, including Rad53, in the control of cell morphology. Checkpoint mutants have aberrant cell morphology and cell walls, and show defective bud site selection. Rad53 shows genetic interactions with septin ring pathway

SUBMITTER: Enserink JM 

PROVIDER: S-EPMC2064673 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature