Checkpoint-independent scaling of the Saccharomyces cerevisiae DNA replication program.

ABSTRACT:

Background

In budding yeast, perturbations that prolong S phase lead to a proportionate delay in the activation times of most origins. The DNA replication checkpoint was implicated in this scaling phenotype, as an intact checkpoint was shown to be required for the delayed activation of late origins in response to hydroxyurea treatment. In support of that, scaling is lost in cells deleted of mrc1, a mediator of the replication checkpoint signal. Mrc1p, however, also plays a role in normal replication.

Results

To examine whether the replication checkpoint is required for scaling the replication profile with S phase duration we measured the genome-wide replication profile of different MRC1 alleles that separate its checkpoint function from its role in normal replication, and fu

SUBMITTER: Gispan A 

PROVIDER: S-EPMC4218987 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature