Identification of a novel testis-specific gene in mice and its potential roles in spermatogenesis.

ABSTRACT:

Aim

Identification of a novel gene in mouse testis and its relation to spermatogenesis.

Methods

Genes expressed during different developmental stages of the mouse testis were screened by DNA microarray. The results of chip analysis were authenticated by reverse transcription-polymerase chain reaction (RT-PCR) technique, as well as the tissue distribution of the selected genes. The characteristics of the selected genes were analyzed by bioinformatics tools.

Results

A novel gene, TSC77, was identified and located at the mouse chromosome 2G1. The full cDNA length of TSC77 was 2280 bp, with a 2046 bp open reading frame encoding a 681 amino acids protein with a predicted molecular weight of 77.17 kDa. The results of subcellular localization of GFP-TSC77 fusion protein indi

SUBMITTER: Tang A 

PROVIDER: S-EPMC2080497 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature