Modulation of interleukin-1 transcriptional response by the interaction between VRK2 and the JIP1 scaffold protein.

ABSTRACT:

Background

Cellular biological responses to specific stimulation are determined by a balance among signaling pathways. Protein interactions are likely to modulate these pathways. Vaccinia-related kinase-2 (VRK2) is a novel human kinase that can modulate different signaling pathways.

Principal findings

We report that in vivo, the activity of JIP1-JNK complexes is downregulated by VRK2 in response to interleukin-1beta. Also the reduction of endogenous VRK2 with shRNA increases the transcriptional response to IL-1beta. The JIP1 scaffold protein assembles three consecutive members of a given MAPK pathway forming signaling complexes and their signal can be modulated by interactions with regulatory proteins that remain to be identified. Knocking-down JIP1 with siRNA resulted in el

SUBMITTER: Blanco S 

PROVIDER: S-EPMC2243017 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature