A functional map of NFkappaB signaling identifies novel modulators and multiple system controls.

ABSTRACT:

Background

The network of signaling pathways that leads to activation of the NFkappaB transcription factors is a branched structure with different inputs and cross-coupling with other signaling pathways. How these signals are integrated to produce specific, yet diverse responses is not clearly understood. To identify the components and structural features of the NFkappaB network, a series of cell-based, genomic screens was performed using a library of approximately 14,500 full-length genes.

Results

A total of 154 positive and 88 negative modulators of NFkappaB signaling were identified. Using a series of dominant-negative constructs and functional assays, these modulators were mapped to the known NFkappaB signaling cascade. Most of the positive modulators acted upstream of t

SUBMITTER: Halsey TA 

PROVIDER: S-EPMC2394752 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature