Terminal restriction fragment length polymorphism for identification of Cryptosporidium species in human feces.

ABSTRACT: Effective management of human cryptosporidiosis requires efficient methods for detection and identification of the species of Cryptosporidium isolates. Identification of isolates to the species level is not routine for diagnostic assessment of cryptosporidiosis, which leads to uncertainty about the epidemiology of the Cryptosporidium species that cause human disease. We developed a rapid and reliable method for species identification of Cryptosporidium oocysts from human fecal samples using terminal restriction fragment polymorphism (T-RFLP) analysis of the 18S rRNA gene. This method generated diagnostic fragments unique to the species of interest. A panel of previously identified isolates of species was blind tested to validate the method, which determined the correct species identity in

SUBMITTER: Waldron LS 

PROVIDER: S-EPMC2612217 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature