Libraries against libraries for combinatorial selection of replicating antigen-antibody pairs.

ABSTRACT: Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody libraries to select high-affinity antibodies to every protein encoded by the genome. However, this is a large task because the selection formats used today for combinatorial antibody libraries are geared toward generating antibodies to one antigen at a time. Here, we describe a method that accelerates the identification of antibodies to a multitude of antigens simultaneously by matching combinatorial antibody libraries against eukaryotic antigen libraries so that replication-competent cognate antigen-antibody pairs can be directly selected. Phage and yeast display systems are used because they

SUBMITTER: Bowley DR 

PROVIDER: S-EPMC2621251 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature