Periodontal therapy alters gene expression of peripheral blood monocytes.

ABSTRACT:

Aims

We investigated the effects of periodontal therapy on gene expression of peripheral blood monocytes.

Methods

Fifteen patients with periodontitis gave blood samples at four time points: 1 week before periodontal treatment (#1), at treatment initiation (baseline, #2), 6-week (#3) and 10-week post-baseline (#4). At baseline and 10 weeks, periodontal status was recorded and subgingival plaque samples were obtained. Periodontal therapy (periodontal surgery and extractions without adjunctive antibiotics) was completed within 6 weeks. At each time point, serum concentrations of 19 biomarkers were determined. Peripheral blood monocytes were purified, RNA was extracted, reverse-transcribed, labelled and hybridized with AffymetrixU133Plus2.0 chips. Expression profiles were analys

SUBMITTER: Papapanou PN 

PROVIDER: S-EPMC2670555 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature