Globaltest and GOEAST: two different approaches for Gene Ontology analysis.

ABSTRACT:

Background

Gene set analysis is a commonly used method for analysing microarray data by considering groups of functionally related genes instead of individual genes. Here we present the use of two gene set analysis approaches: Globaltest and GOEAST.Globaltest is a method for testing whether sets of genes are significantly associated with a variable of interest. GOEAST is a freely accessible web-based tool to test GO term enrichment within given gene sets. The two approaches were applied in the analysis of gene lists obtained from three different contrasts in a microarray experiment conducted to study the host reactions in broilers following Eimeria infection.

Results

The Globaltest identified significantly associated gene sets in one of the three contrasts made in the microa

SUBMITTER: Hulsegge I 

PROVIDER: S-EPMC2712740 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature