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Generation of pluripotent stem cells from patients with type 1 diabetes.

ABSTRACT: Type 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic beta cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallmarks of pluripotency and can be differentiated into insulin-producing cells. These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy.

SUBMITTER: Maehr R

PROVIDER: S-EPMC2735559 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

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Generation of pluripotent stem cells from patients with type 1 diabetes.

Maehr René R Chen Shuibing S Snitow Melinda M Ludwig Thomas T Yagasaki Lisa L Goland Robin R Leibel Rudolph L RL Melton Douglas A DA

Proceedings of the National Academy of Sciences of the United States of America 20090831 37

Type 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic beta cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallm ...[more]

PMID: 19720998

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