Ontology highlight
ABSTRACT:
SUBMITTER: Finkbeiner SR
PROVIDER: S-EPMC2753140 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Journal of virology 20090812 20
The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides. ...[more]