An approach to comparing tiling array and high throughput sequencing technologies for genomic transcript mapping.

ABSTRACT:

Background

There are two main technologies for transcriptome profiling, namely, tiling microarrays and high-throughput sequencing. Recently there has been a tremendous amount of excitement about the latter because of the advent of next-generation sequencing technologies and its promises. Consequently, the question of the moment is how these two technologies compare. Here we attempt to develop an approach to do a fair comparison of transcripts identified from tiling microarray and MPSS sequencing data.

Findings

This comparison is a challenging task because the sequencing data is discrete while the tiling array data is continuous. We use the published rice and Arabidopsis datasets which provide currently best matched sets of arrays and sequencing experiments using a slightly e

SUBMITTER: Sasidharan R 

PROVIDER: S-EPMC2764720 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature