Transcriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons.

ABSTRACT:

Background

Trypanosome gene expression is regulated almost exclusively at the post-transcriptional level, with mRNA degradation playing a decisive role. When trypanosomes are transferred from the blood of a mammal to the midgut of a Tsetse fly, they transform to procyclic forms: gene expression is reprogrammed, changing the cell surface and switching the mode of energy metabolism. Within the blood, trypanosomes can pre-adapt for Tsetse transmission, becoming growth-arrested stumpy forms. We describe here the transitions in gene expression that occur during differentiation of in-vitro cultured bloodstream forms to procyclic forms.

Results

Some mRNAs showed changes within 30 min of cis-aconitate addition, whereas others responded 12-24 hours later. For the first 12 h after add

SUBMITTER: Queiroz R 

PROVIDER: S-EPMC2772864 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature