Release of ATP from rat urinary bladder mucosa: role of acid, vanilloids and stretch.

ABSTRACT:

Background and purpose

ATP, released from urothelial cells, modulates afferent nerve firing from the urinary bladder. Here, we have characterized ATP release from the rat bladder mucosa in response to acid, capsaicin, electrical field stimulation (EFS) and stretch, using agonists and antagonists at transient receptor potential vanilloid receptor 1 (TRPV1) and acid-sensing ion channels (ASICs).

Experimental approach

Rat mucosal strips (containing urothelium and lamina propria) in Perspex microbaths were superfused with Krebs solution. ATP was measured after exposure of matched strips to acid (pH 6.6-5.0), capsaicin (0.1-10 microM), EFS or stretch (150% of original length).

Key results

Median basal ATP release was 3.46 nmol g(-1). The mucosal strips responded to stimuli

SUBMITTER: Sadananda P 

PROVIDER: S-EPMC2801206 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature