Ectopic expression of E2F1 stimulates beta-cell proliferation and function.

ABSTRACT:

Objective

Generating functional beta-cells by inducing their proliferation may provide new perspectives for cell therapy in diabetes. Transcription factor E2F1 controls G(1)- to S-phase transition during the cycling of many cell types and is required for pancreatic beta-cell growth and function. However, the consequences of overexpression of E2F1 in beta-cells are unknown.

Research design and methods

The effects of E2F1 overexpression on beta-cell proliferation and function were analyzed in isolated rat beta-cells and in transgenic mice.

Results

Adenovirus AdE2F1-mediated overexpression of E2F1 increased the proliferation of isolated primary rat beta-cells 20-fold but also enhanced beta-cell death. Coinfection with adenovirus AdAkt expressing a constitutively active f

SUBMITTER: Grouwels G 

PROVIDER: S-EPMC2874704 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature