Transcriptome analysis in non-model species: a new method for the analysis of heterologous hybridization on microarrays.

ABSTRACT:

Background

Recent developments in high-throughput methods of analyzing transcriptomic profiles are promising for many areas of biology, including ecophysiology. However, although commercial microarrays are available for most common laboratory models, transcriptome analysis in non-traditional model species still remains a challenge. Indeed, the signal resulting from heterologous hybridization is low and difficult to interpret because of the weak complementarity between probe and target sequences, especially when no microarray dedicated to a genetically close species is available.

Results

We show here that transcriptome analysis in a species genetically distant from laboratory models is made possible by using MAXRS, a new method of analyzing heterologous hybridization on micro

SUBMITTER: Degletagne C 

PROVIDER: S-EPMC2901317 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature