Cross-species hybridization of microarrays for studying tumor transcriptome of brain metastasis.

ABSTRACT: Although the importance of the cellular microenvironment (soil) during invasion and metastasis of cancer cells (seed) has been well-recognized, technical challenges have limited the ability to assess the influence of the microenvironment on cancer cells at the molecular level. Here, we show that an experimental strategy, competitive cross-species hybridization of microarray experiments, can characterize the influence of different microenvironments on cancer cells by independently extracting gene expression data of cancer and host cells when human cancer cells were xenografted into different organ sites of immunocompromised mice. Surprisingly, the analysis of gene expression data showed that the brain microenvironment induces complete reprogramming of metastasized cancer cells, resulting in

SUBMITTER: Park ES 

PROVIDER: S-EPMC3198333 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature