Cell-type-specific long-range looping interactions identify distant regulatory elements of the CFTR gene.

ABSTRACT: Identification of regulatory elements and their target genes is complicated by the fact that regulatory elements can act over large genomic distances. Identification of long-range acting elements is particularly important in the case of disease genes as mutations in these elements can result in human disease. It is becoming increasingly clear that long-range control of gene expression is facilitated by chromatin looping interactions. These interactions can be detected by chromosome conformation capture (3C). Here, we employed 3C as a discovery tool for identification of long-range regulatory elements that control the cystic fibrosis transmembrane conductance regulator gene, CFTR. We identified four elements in a 460-kb region around the locus that loop specifically to the CFTR promoter exc

SUBMITTER: Gheldof N 

PROVIDER: S-EPMC2910055 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature