Amplification-free digital gene expression profiling from minute cell quantities.

ABSTRACT: Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.

SUBMITTER: Ozsolak F 

PROVIDER: S-EPMC2975601 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature